Skip to content

Scientific Figures

Cell and Molecular Biology

  • Antibodies
  • Recombinant proteins
  • Rabbit Monoclonal Antibodies
  • Rabbit Polyclonal antibodies
  • Coronavirus and Covid-19
  • Scientific Figures Privacy Policy
  • Delivery of superoxide dismutase by TAT and Abalone peptides for the protection of skin cells against oxidative stress
  • Distributors
  • Toggle search form

DNA-based detection of grapevine trunk-disease pathogens from environmental spore samples

Posted on February 8, 2022 By Matthew No Comments on DNA-based detection of grapevine trunk-disease pathogens from environmental spore samples
In California vineyards, spore dispersal of fungi that cause grapevine trunk diseases Botryosphaeria dieback and Eutypa dieback occurs with winter rains. Spores infect through pruning wounds made to the woody structure of the vine in winter. Better timing of preventative practices that minimize infection may benefit from routine spore-trapping, which could pinpoint site-specific time frames of spore dispersal. To speed pathogen detection from environmental spore samples, we identified species-specific PCR primers and protocols. Then we compared the traditional culture-based method versus our new DNA-based method.
PCR primers for Botryosphaeria-dieback pathogen Neofusicoccum parvum and Eutypa-dieback pathogen Eutypa lata were confirmed species-specific, through extensive testing of related species (in families Botryosphaeriaceae and Diatrypaceae, respectively), other trunk-disease pathogens, and saprophytic fungi that sporulate in vineyards.
Consistent detection of N. parvum was achieved from spore suspensions used fresh or stored at -20°C, whereas consistent detection of E. lata was achieved only with a new spore-lysis method, using zirconia/silica beads in a FastPrep homogenizer (MP Biomedicals; Solon, Ohio, USA), and only from spore suspensions used fresh. Freezing E. lata spores at -20°C made detection inconsistent.•From environmental samples, spores of E. lata were detected only via PCR, whereas spores of N. parvum were detected both via PCR and in culture.

Elucidating biofilm diversity on water lily leaves through 16S rRNA amplicon analysis: Comparison of four DNA extraction kits

Premise: Within a broader study on leaf fossilization in freshwater environments, a long-term study on the development and microbiome composition of biofilms on the foliage of aquatic plants has been initiated to understand how microbes and biofilms contribute to leaf decay and preservation.
Here, water lily leaves are employed as a study model to investigate the relationship between bacterial microbiomes, biodegradation, and fossilization. We compare four DNA extraction kits to reduce biases in interpretation and to identify the most suitable kit for the extraction of DNA from bacteria associated with biofilms on decaying water lily leaves for 16S rRNA amplicon analysis.
Methods: We extracted surface-associated DNA from Nymphaea leaves in early stages of decay at two water depth levels using four commercially available kits to identify the most suitable protocol for bacterial extraction, applying a mock microbial community standard to enable a reliable comparison of the kits.
Results: Kit 4, the FastDNA Spin Kit for Soil, resulted in high DNA concentrations with better quality and yielded the most accurate depiction of the mock community. Comparison of the leaves at two water depths showed no significant differences in community composition.
Discussion: The success of Kit 4 may be attributed to its use of bead beating with a homogenizer, which was more efficient in the lysis of Gram-positive bacteria than the manual vortexing protocols used by the other kits. Our results show that microbial composition on leaves during early decay remains comparable and may change only in Bio Basic Bead Homogenizers later stages of decomposition.

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Intraocular bacterial infections are a danger to the vision. Researchers use animal models to investigate the host and bacterial factors and immune response pathways associated with infection to identify viable therapeutic targets and to test drugs to prevent blindness. The intravitreal injection technique is used to inject organisms, drugs, or other substances directly into the vitreous cavity in the posterior segment of the eye.
Here, we demonstrated this injection technique to initiate infection in the mouse eye and the technique of quantifying intraocular bacteria. Bacillus cereus was grown in brain heart infusion liquid media for 18 hours and resuspended to a concentration 100 colony forming units (CFU)/0.5 µL. A C57BL/6J mouse was anesthetized using a combination of ketamine and xylazine. Using a picoliter microinjector and glass capillary needles, 0.5 µL of the Bacillus suspension was injected into the mid vitreous of the mouse eye.
The contralateral control eye was either injected with sterile media (surgical control) or was not injected (absolute control). At 10 hours post infection, mice were euthanized, and eyes were harvested using sterile surgical tweezers and placed into a tube containing 400 µL sterile PBS and 1 mm sterile glass beads. For ELISAs or myeloperoxidase assays, proteinase inhibitor was added to the tubes. For RNA extraction, the appropriate lysis buffer was added. Eyes were homogenized in a tissue homogenizer for 1-2 minutes.
Homogenates were serially diluted 10-fold in PBS and track diluted onto agar plates. The remainder of the homogenates were stored at -80 °C for additional assays. Plates were incubated for 24 hours and CFU per eye was quantified. These techniques result in reproducible infections in mouse eyes and facilitate quantitation of viable bacteria, the host immune response, and omics of host and bacterial gene expression.

Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization

This chapter covers the various methods of mechanical cell disruption and tissue homogenization that are currently commercially available for processing small samples s < 1 mL) to larger multikilogram production quantities. These mechanical methods of lysing do not introduce chemicals or enzymes to the system. However, the energies required when using these “harsh,” high mechanical energy methods can be enough to damage the very components being sought.
The destruction of cell membranes and walls is effected by subjecting the cells (a) to shearing by liquid flow, (b) to exploding by pressure differences between inside and outside of cell, (c) to collision forces by impact of beads or paddles, or (d) a combination of these forces.Practical suggestions to optimize each method, where to acquire such equipment, and links to reference sources are included. Several novel technologies are presented.

Proteomic evaluation of plasma membrane fraction prepared from mouse liver and kidney using a bead homogenizer: Enrichment of drug-related transporter proteins

Quantifying the protein levels of drug transporters in plasma membrane fraction helps elucidate the function of these transporters. In this study, we conducted a proteomic evaluation of enriched drug-related transporter proteins in plasma membrane fraction prepared from mouse liver and kidney tissues using the Membrane Protein Extraction Kit and a bead homogenizer. Crude and plasma membrane fractions were prepared using either the Dounce or bead homogenizer, and protein levels were determined using quantitative proteomics.
In liver tissues, the plasma membrane fractions were more enriched in transporter proteins than the crude membrane fractions; the average enrichment ratios of plasma-to-crude membrane fractions were 3.31 and 6.93 using the Dounce and bead homogenizers, respectively. The concentrations of transporter proteins in plasma membrane fractions determined using the bead homogenizer were higher than those determined using the Dounce homogenizer.
Meanwhile, in kidney tissues, the plasma membrane fractions were enriched in transporters localized in the brush-border membrane to the same degree for both the homogenizers; however, the membrane fractions obtained using either homogenizer were not enriched in Na+/K+-ATPase and transporters localized in the basolateral membrane. These results indicate that fractionation, using the bead homogenizer, yielded transporter-enriched plasma membrane fractions from mouse liver and kidney tissues; however, no enrichment of basolateral transporters was observed in plasma membrane fractions prepared from kidney tissues.

Micro Bead Sterlizer

B1201-E Benchmark Scientific 1 PC 542.7 EUR

Micro Bead Sterlizer

B1202-E Benchmark Scientific 1 PC 84.14 EUR

Refill Glass Beads

B1201-BEAD Benchmark Scientific 1 PC 117.78 EUR

Bangs Lab Bead Solution

SOLN1-1000 Bangs Laboratories 1000 ML 155.06 EUR

Bangs Lab Bead Solution

SOLN1-2000 Bangs Laboratories 2000 ML 212.7 EUR

Bangs Lab Bead Solution

SOLN1-500 Bangs Laboratories 500 ML 98.51 EUR

Bead Sample Pack - 50mL Set

BSP-50B2 Next Advance 1pack 404 EUR

Bead Sample Pack - 5E Set

BSP-5E Next Advance 1pack 252 EUR

Bead Sample Pack - 5M Set

BSP-5Y Next Advance 1pack 241 EUR

Bead Sample Pack - Full Set

BSP-ALL2 Next Advance 1pack 384 EUR

Bead Sample Pack - Cell Cultures

BSP-CC2 Next Advance 1pack 195 EUR

Bead Sample Pack - Microcentrifuge Set

BSP-MC2 Next Advance 1pack 232 EUR

Bead Sample Pack - Organ Tissues

BSP-OT3 Next Advance 1pack 235 EUR

CM Rapid Run Agarose Bead

CMRR-25 ABTBeads 25 ml 102 EUR

CM Rapid Run Agarose Bead

CMRR-300 ABTBeads 300 ml 492 EUR

DEAE Rapid Run Agarose Bead

DEAERR-25 ABTBeads 25 ml 102 EUR

DEAE Rapid Run Agarose Bead

DEAERR-300 ABTBeads 300 ml 492 EUR

SP Rapid Run Agarose Bead

SPRR-25 ABTBeads 25 ml 102 EUR

SP Rapid Run Agarose Bead

SPRR-300 ABTBeads 300 ml 492 EUR

Anti-DDK(FLAG) Agarose bead Conjugated

D4501-001 GenDepot 1ml 550 EUR

Anti-DDK(FLAG) Agarose bead Conjugated

D4501-005 GenDepot 5ml 1373 EUR

Nickel NTA Magnetic Agarose Bead (5%)

MagNTANi5-10 ABTBeads 10ml suspension 284 EUR

Nickel NTA Magnetic Agarose Bead (5%)

MagNTANi5-2 ABTBeads 2ml suspension 99 EUR

Nickel NTA Magnetic Agarose Bead (5%)

MagNTANi5-5 ABTBeads 5ml suspension 174 EUR

Protonex™ Red 600-Latex Bead Conjugate

21209 AAT Bioquest 1 mL 306 EUR

Bangs Lab Bead Coupling Buffer, pH 4.5

BUFF1-1000 Bangs Laboratories 1000 ML 155.06 EUR

Bangs Lab Bead Coupling Buffer, pH 4.5

BUFF1-2000 Bangs Laboratories 2000 ML 212.7 EUR

Bangs Lab Bead Coupling Buffer, pH 4.5

BUFF1-250 Bangs Laboratories 250 ML 87.64 EUR
Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data, Disseminating Research Outputs: The CrowdHEALTH Project Tags:dna ancestry, dna bts, dna definition, dna genetics, dna hr block, dna hrblock login, dna hrblock login employee, dna lyrics, dna methylation, dna molecule, dna painter, dna polymerase, dna replication, dna rna, dna sequencing, dna stock, dna structure, dna testing, dna tests, dna transcription, dna.hrblock.com, dnajlion7 rumble, dnase, proteins are made of, proteins are made where in the cell, proteins are polymers composed of, proteins are polymers of, proteins biology, proteins definition, proteins elements, proteins example science project, proteins examples, proteins examples biology, proteins foods, proteins function, proteins monomer, proteins play in the body, proteins structure, proteinsimple, proteinsimple milo, proteinska dijeta, proteinske palačinke, proteinski kruh, proteinsplus, proteinstruktur, proteinsyntesen enkelt forklart, proteinsynthese

Post navigation

Previous Post: Chemically modified mycological materials having absorbent properties
Next Post: Free Time-to-Digital Converter for Home-Monitoring LiDAR Sensors

Related Posts

Analyzing occupational heat stress using sensor-based monitoring: a wearable approach with environmental ergonomics perspective Disseminating Research Outputs: The CrowdHEALTH Project
Gadgets and Armamentarium of Maxillofacial Surgeons during Coronavirus Pandemic Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data
CRYO FREEZERS FROM ARCTIKO Disseminating Research Outputs: The CrowdHEALTH Project
Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data
Genetic analysis of prokaryotic and eukaryotic DNA-binding proteins in Escherichia coli Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data
Chemically modified mycological materials having absorbent properties Disseminating Research Outputs: The CrowdHEALTH Project

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

GRIP MolecularFollow

Next Generation graphene bio-sensors enabling lab quality diagnostics ... at home

GRIP Molecular
GripMolecularGRIP Molecular@GripMolecular·
17 May

Don't feel good? Stuck at home? New GRIP Micro Chip technology could arm sick people with the power and precision of a big box centralized laboratory ... on a device that fits in your pocket and goes anywhere you do. #telehealth #healthtech #homecare

https://youtu.be/tKrLI20ItWM

Reply on Twitter 1526369148166672384Retweet on Twitter 1526369148166672384Like on Twitter 15263691481666723841Twitter 1526369148166672384
GripMolecularGRIP Molecular@GripMolecular·
8 May

University of Minnesota developed a smartphone powered diagnostic microchip ... paving the way for more convenient, affordable and comprehensive at-home medical testing.
#telemedicine #homecare #healthtech
https://tinyurl.com/527jtp6u

Reply on Twitter 1523400696246472704Retweet on Twitter 1523400696246472704Like on Twitter 1523400696246472704Twitter 1523400696246472704
GripMolecularGRIP Molecular@GripMolecular·
14 Mar

As featured on GenomeWeb 360Dx: "Grip Molecular Developing Biosensor Panel to Detect SARS-CoV-2, Other Respiratory Infections ... " Link to full article: https://lnkd.in/dSXfq6se
#sarscov2 #healthtech #hometest

Reply on Twitter 1503457813175054339Retweet on Twitter 1503457813175054339Like on Twitter 15034578131750543391Twitter 1503457813175054339
Load More...

Recent Posts

  • Real time kinetic flow cytometry measurements of cellular parameter changes evoked by nanosecond pulsed electric field
  • A CMOS Integrator-Based Clock-Free Time-to-Digital Converter for Home-Monitoring LiDAR Sensors
  • Prudent public health intervention strategies to control the coronavirus disease 2019 transmission in India: A mathematical model-based approach
  • Gadgets and Armamentarium of Maxillofacial Surgeons during Coronavirus Pandemic
  • Analyzing occupational heat stress using sensor-based monitoring: a wearable approach with environmental ergonomics perspective

Meta

  • Log in
  • Entries feed
  • Comments feed
  • WordPress.org

Copyright © 2022 Scientific Figures.

Powered by PressBook Grid Blogs theme