Skip to content

Scientific Figures

Cell and Molecular Biology

  • Antibodies
  • Recombinant proteins
  • Rabbit Monoclonal Antibodies
  • Rabbit Polyclonal antibodies
  • Coronavirus and Covid-19
  • Scientific Figures Privacy Policy
  • Delivery of superoxide dismutase by TAT and Abalone peptides for the protection of skin cells against oxidative stress
  • Distributors
  • Toggle search form

DNA-based detection of grapevine trunk-disease pathogens from environmental spore samples

Posted on February 8, 2022 By Matthew No Comments on DNA-based detection of grapevine trunk-disease pathogens from environmental spore samples
In California vineyards, spore dispersal of fungi that cause grapevine trunk diseases Botryosphaeria dieback and Eutypa dieback occurs with winter rains. Spores infect through pruning wounds made to the woody structure of the vine in winter. Better timing of preventative practices that minimize infection may benefit from routine spore-trapping, which could pinpoint site-specific time frames of spore dispersal. To speed pathogen detection from environmental spore samples, we identified species-specific PCR primers and protocols. Then we compared the traditional culture-based method versus our new DNA-based method.
PCR primers for Botryosphaeria-dieback pathogen Neofusicoccum parvum and Eutypa-dieback pathogen Eutypa lata were confirmed species-specific, through extensive testing of related species (in families Botryosphaeriaceae and Diatrypaceae, respectively), other trunk-disease pathogens, and saprophytic fungi that sporulate in vineyards.
Consistent detection of N. parvum was achieved from spore suspensions used fresh or stored at -20°C, whereas consistent detection of E. lata was achieved only with a new spore-lysis method, using zirconia/silica beads in a FastPrep homogenizer (MP Biomedicals; Solon, Ohio, USA), and only from spore suspensions used fresh. Freezing E. lata spores at -20°C made detection inconsistent.•From environmental samples, spores of E. lata were detected only via PCR, whereas spores of N. parvum were detected both via PCR and in culture.

Elucidating biofilm diversity on water lily leaves through 16S rRNA amplicon analysis: Comparison of four DNA extraction kits

Premise: Within a broader study on leaf fossilization in freshwater environments, a long-term study on the development and microbiome composition of biofilms on the foliage of aquatic plants has been initiated to understand how microbes and biofilms contribute to leaf decay and preservation.
Here, water lily leaves are employed as a study model to investigate the relationship between bacterial microbiomes, biodegradation, and fossilization. We compare four DNA extraction kits to reduce biases in interpretation and to identify the most suitable kit for the extraction of DNA from bacteria associated with biofilms on decaying water lily leaves for 16S rRNA amplicon analysis.
Methods: We extracted surface-associated DNA from Nymphaea leaves in early stages of decay at two water depth levels using four commercially available kits to identify the most suitable protocol for bacterial extraction, applying a mock microbial community standard to enable a reliable comparison of the kits.
Results: Kit 4, the FastDNA Spin Kit for Soil, resulted in high DNA concentrations with better quality and yielded the most accurate depiction of the mock community. Comparison of the leaves at two water depths showed no significant differences in community composition.
Discussion: The success of Kit 4 may be attributed to its use of bead beating with a homogenizer, which was more efficient in the lysis of Gram-positive bacteria than the manual vortexing protocols used by the other kits. Our results show that microbial composition on leaves during early decay remains comparable and may change only in Bio Basic Bead Homogenizers later stages of decomposition.

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Intraocular bacterial infections are a danger to the vision. Researchers use animal models to investigate the host and bacterial factors and immune response pathways associated with infection to identify viable therapeutic targets and to test drugs to prevent blindness. The intravitreal injection technique is used to inject organisms, drugs, or other substances directly into the vitreous cavity in the posterior segment of the eye.
Here, we demonstrated this injection technique to initiate infection in the mouse eye and the technique of quantifying intraocular bacteria. Bacillus cereus was grown in brain heart infusion liquid media for 18 hours and resuspended to a concentration 100 colony forming units (CFU)/0.5 µL. A C57BL/6J mouse was anesthetized using a combination of ketamine and xylazine. Using a picoliter microinjector and glass capillary needles, 0.5 µL of the Bacillus suspension was injected into the mid vitreous of the mouse eye.
The contralateral control eye was either injected with sterile media (surgical control) or was not injected (absolute control). At 10 hours post infection, mice were euthanized, and eyes were harvested using sterile surgical tweezers and placed into a tube containing 400 µL sterile PBS and 1 mm sterile glass beads. For ELISAs or myeloperoxidase assays, proteinase inhibitor was added to the tubes. For RNA extraction, the appropriate lysis buffer was added. Eyes were homogenized in a tissue homogenizer for 1-2 minutes.
Homogenates were serially diluted 10-fold in PBS and track diluted onto agar plates. The remainder of the homogenates were stored at -80 °C for additional assays. Plates were incubated for 24 hours and CFU per eye was quantified. These techniques result in reproducible infections in mouse eyes and facilitate quantitation of viable bacteria, the host immune response, and omics of host and bacterial gene expression.

Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization

This chapter covers the various methods of mechanical cell disruption and tissue homogenization that are currently commercially available for processing small samples s < 1 mL) to larger multikilogram production quantities. These mechanical methods of lysing do not introduce chemicals or enzymes to the system. However, the energies required when using these “harsh,” high mechanical energy methods can be enough to damage the very components being sought.
The destruction of cell membranes and walls is effected by subjecting the cells (a) to shearing by liquid flow, (b) to exploding by pressure differences between inside and outside of cell, (c) to collision forces by impact of beads or paddles, or (d) a combination of these forces.Practical suggestions to optimize each method, where to acquire such equipment, and links to reference sources are included. Several novel technologies are presented.

Proteomic evaluation of plasma membrane fraction prepared from mouse liver and kidney using a bead homogenizer: Enrichment of drug-related transporter proteins

Quantifying the protein levels of drug transporters in plasma membrane fraction helps elucidate the function of these transporters. In this study, we conducted a proteomic evaluation of enriched drug-related transporter proteins in plasma membrane fraction prepared from mouse liver and kidney tissues using the Membrane Protein Extraction Kit and a bead homogenizer. Crude and plasma membrane fractions were prepared using either the Dounce or bead homogenizer, and protein levels were determined using quantitative proteomics.
In liver tissues, the plasma membrane fractions were more enriched in transporter proteins than the crude membrane fractions; the average enrichment ratios of plasma-to-crude membrane fractions were 3.31 and 6.93 using the Dounce and bead homogenizers, respectively. The concentrations of transporter proteins in plasma membrane fractions determined using the bead homogenizer were higher than those determined using the Dounce homogenizer.
Meanwhile, in kidney tissues, the plasma membrane fractions were enriched in transporters localized in the brush-border membrane to the same degree for both the homogenizers; however, the membrane fractions obtained using either homogenizer were not enriched in Na+/K+-ATPase and transporters localized in the basolateral membrane. These results indicate that fractionation, using the bead homogenizer, yielded transporter-enriched plasma membrane fractions from mouse liver and kidney tissues; however, no enrichment of basolateral transporters was observed in plasma membrane fractions prepared from kidney tissues.

Ceramic grinding bars 3/8X5/8, 45°, angled medium ceramic homogenizers pack of 100

IPD9600-3858-1 Benchmark Scientific each 39.14 EUR

Ceramic grinding bars 3/8X7/8 , 45°, angled medium ceramic homogenizers pack of 100

IPD9600-3878-1 Benchmark Scientific each 39.14 EUR

BeadBug™ Microtube homogenizer, 115V

D1030 Benchmark Scientific 1 each 1000.42 EUR

BeadBug™ Microtube homogenizer, 230V

D1030-E Benchmark Scientific 1 PC 1000.42 EUR

BeadBug 6, Six Position Homogenizer, 115V

D1036 Benchmark Scientific 1 each 2696.23 EUR

BeadBug 6, Six Position Homogenizer, 230V

D1036-E Benchmark Scientific 1 PC 2696.23 EUR

BeadBug 6 Six Position Homogenizer 230V - EACH

HOM3018 Scientific Laboratory Supplies EACH 3825.9 EUR

BeadBlaster 96 Ball Mill Homogenizer, 120V US Plug

IPD9600 Benchmark Scientific each 11892.18 EUR

BeadBlaster 96 Ball Mill Homogenizer, 230V EU Plug

IPD9600-E Benchmark Scientific each 11892.18 EUR

BeadBlaster™ Microtube homogenizer, 115V

D2400 Benchmark Scientific 1 each 9460.6 EUR

BeadBlaster™ Microtube homogenizer, 230V

D2400-E Benchmark Scientific 1 PC 9460.6 EUR

BeadBlaster Microtube homogenizer 230V - EACH

HOM3012 Scientific Laboratory Supplies EACH 13678.2 EUR

BeadBug Microtube homogenizer - EACH

SLS1402 Scientific Laboratory Supplies EACH 1629.45 EUR

BeadBlaster™ 24 Refrigerated Microtube Homogenizer, 115V

D2400-R Benchmark Scientific 1 each 15098.14 EUR

BeadBlaster™ 24 Refrigerated Microtube Homogenizer, 230V

D2400-R-E Benchmark Scientific 1 each 15098.14 EUR

BeadBlaster 24 Refrigerated Microtube Homogenizer 230V - EACH

HOM3078 Scientific Laboratory Supplies EACH 24792.75 EUR

Homogenizer stand for Agile™ Hand-held homogenizer

AHM1 ACTGene VS 414.21 EUR

Homogenizer stand for Agile? Hand-held homogenizer

AHM1-VS ACTGene each 634.8 EUR

Microtube homogenizer, 115V

BCM1200 Bio Basic 1 pcs, 1 UNIT 11944.61 EUR

Microtube homogenizer, 115V

BCM1201 Bio Basic 1 pcs, 1 UNIT 1224.14 EUR

HOMOGENIZER

H291 PhytoTechnology Laboratories 1EA 739.98 EUR

SpeedMill PLUS, Homogenizer 220 V

AJ845-00007-2 Westburg each 11118 EUR

Nail Homogenizer

099CE2000 Glascol each 450 EUR

TUBE, HOMOGENIZER (25/PACK)

H293 PhytoTechnology Laboratories 1EA 346.67 EUR

Dounce Tissue Homogenizer

1998-1 Biovision each 470.4 EUR

Homogenizer with plain pestle (P.P.) 2

GW124-1NO EWC Diagnostics 1 unit 18.02 EUR

Homogenizer with plain pestle (P.P.) 5

GW125-1NO EWC Diagnostics 1 unit 18.02 EUR

Homogenizer with plain pestle (P.P.) 10

GW126-1NO EWC Diagnostics 1 unit 24.28 EUR
×
Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data, Disseminating Research Outputs: The CrowdHEALTH Project Tags:dna ancestry, dna bts, dna definition, dna genetics, dna hr block, dna hrblock login, dna hrblock login employee, dna lyrics, dna methylation, dna molecule, dna painter, dna polymerase, dna replication, dna rna, dna sequencing, dna stock, dna structure, dna testing, dna tests, dna transcription, dna.hrblock.com, dnajlion7 rumble, dnase, proteins are made of, proteins are made where in the cell, proteins are polymers composed of, proteins are polymers of, proteins biology, proteins definition, proteins elements, proteins example science project, proteins examples, proteins examples biology, proteins foods, proteins function, proteins monomer, proteins play in the body, proteins structure, proteinsimple, proteinsimple milo, proteinska dijeta, proteinske palačinke, proteinski kruh, proteinsplus, proteinstruktur, proteinsyntesen enkelt forklart, proteinsynthese

Post navigation

Previous Post: Chemically modified mycological materials having absorbent properties
Next Post: Free Time-to-Digital Converter for Home-Monitoring LiDAR Sensors

Related Posts

Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data
Free Time-to-Digital Converter for Home-Monitoring LiDAR Sensors Disseminating Research Outputs: The CrowdHEALTH Project
Chemically modified mycological materials having absorbent properties Disseminating Research Outputs: The CrowdHEALTH Project
CRYO FREEZERS FROM ARCTIKO Disseminating Research Outputs: The CrowdHEALTH Project
Gadgets and Armamentarium of Maxillofacial Surgeons during Coronavirus Pandemic Air Pollution and Health: The Need for a Medical Reading of Environmental Monitoring Data
Prudent public health intervention strategies to control the coronavirus disease 2019 transmission in India: A mathematical model-based approach Disseminating Research Outputs: The CrowdHEALTH Project

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

GripMolecular Follow

@ ·
now

Reply on Twitter Retweet on Twitter Like on Twitter Twitter
Load More

Recent Posts

  • NIH References of Lab reagents
  • Affigen reagent of Lab rec.
  • Compare Pcr lab reagents for research
  • Compare recombinant lab reagents for research
  • Compare Appoptosis lab reagents for research

Meta

  • Log in
  • Entries feed
  • Comments feed
  • WordPress.org

Copyright © 2025 Scientific Figures.

Powered by PressBook Grid Blogs theme